Technologieangebote

The site-directed modification of e.g. biomolecules is often time consuming and laborious. This invention provides a high yielding and highly specific method to link nearly any molecule to nearly any other molecule. The “Click reaction” used is the well known Diels-Alder reaction with inverse electron demand. In this fast reaction an appropriate, easily functionalized dien (e.g. Tetrazin) is reacted with an appropriate functionalized dienophil (e.g. Olefin) to yield the conjugate. The release of nitrogen makes the reaction irreversible. No side reactions occur. Further the Intellectual Property covers specific information regarding the ligation of a diagnostic marker AND a therapeutic agent AND a biomolecule. Convincing synthetic data are available for the ligation of peptide with peptide, peptide with marker molecule, peptide with therapeutic agent, peptide with oligo nucleotide, dendritic structure and specific surface modification by means of this technology.

High speed imaging in single spot laser scanning imaging is limited by saturation effects of the fluorescence process in the sample as well as by destructive processes due to the high intensity in the focal spot. The only way to overcome that limitation is to use multiple spots at the same time. We developed a Multi-Photon Multi-Spot Scanning Device which is applicable especially in laser scanning devices such as confocal laser scanners and multi-photon microscopes. It can be used to speed up imaging of living cells which would be harmed by a stronger single beam.

DKFZ developed an optical imaging detector for fluorescence and bioluminescence in small animal imaging, which is compatible for positron electron tomography (PET). Compatibility of light detection with PET has been accomplished by developing an optical detector that consists of a 25 mm x 100 mm photon sensor (liquid cooled) for light detection, a microlens array for field-of-view definition, a septum masks for cross-talk suppression, and a transferable filter for wavelength selection.

This invention describes a simple and robust navigation aid for physicians in bronchoscopy or similar interventional surgery (angiography; implanting of vessel stents as well as cardiac pacemaker). The interventional device can be monitored and in real time and at a defined localized spot a biopsy can be taken. It improves accuracy also in the case of movement of the organ structures in opposite to established procedures. A prototype phantom has been tested successfully at the DKFZ together with the software.

Optical fluorescence and gamma-labeled radio-pharmaceutical imaging offer complementary ways of probing and assessing the physiology of small animals. With the increasing use of both methods for functional molecular imaging in small animal models for applications such as cancer research and drug development, it has become more important to integrate their results, particularly for studies of the same animal. This can be performed through image registration of separate studies or through simultaneous tomographic acquisition with implicit registration of the two imaging modalities. DKFZ developed an optical imaging detector for fluorescence and bioluminescence in small animal imaging, which is compatible for single-photon emission computed tomography (SPECT).

New method for producing a highly diverse antibody library, where pro-teins/antibodies can be selected for specific applications. A large and, compared from cell to cell, highly uniform number of antibodies is covalently attached to the surface of a eukaryotic cell. Using this technology, specific antibodies can be selected together with the presenting cell out of a high number of cells in a comparatively easy way. The technology can also be used humanizing a hybridoma cell line by enabling the homologous recombination of the constant do-mains of human immunoglobulins with the respective gene.